CCDC124 Polyclonal antibody
CCDC124 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 27106-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, human placenta tissue, mouse liver tissue |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 27106-1-AP is CCDC124 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CCDC124 fusion protein Ag25930 相同性解析による交差性が予測される生物種 |
Full Name | coiled-coil domain containing 124 |
Observed molecular weight | 30-32 kDa |
GenBank accession number | BC013949 |
Gene symbol | CCDC124 |
Gene ID (NCBI) | 115098 |
RRID | AB_2880758 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCDC124 is a novel centrosomal and midbody protein that is highly conserved among species. It is ubiquitously expressed with high expression in brain, placenta, liver, spleen, and prostate. At telophase, CCDC124 dissociates from centrosomes and relocalizes to the midzone, subsequently accumulating at the midbody at cytokinesis. It interacts with the Ras-guanine nucleotide exchange factor 1B (RasGEF1B) and is involved in cytokinesis.
Protocols
Product Specific Protocols | |
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WB protocol for CCDC124 antibody 27106-1-AP | Download protocol |
IF protocol for CCDC124 antibody 27106-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Protein J Live Cell Protein Imaging of Tandem Complemented-GFP11-Tagged Coiled-Coil Domain-Containing Protein-124 Identifies this Factor in G3BP1-Induced Stress-Granules |