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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC124 Polyclonal antibody

CCDC124 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27106-1-AP

Synonyms

CCDC124

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using CCDC124 Polyclonal antibody (27106-1-AP)

    WB analysis of mouse brain using 27106-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CCDC124 Polyclonal antibody (27106-1-AP)

    WB analysis of mouse liver using 27106-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using CCDC124 Polyclonal antibody (27106-1-AP)

    WB analysis of human placenta using 27106-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC124 Polyclonal antibody (27106-1-AP)

    IF Staining of HeLa using 27106-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 27106-1-AP (CCDC124 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC124 Polyclonal antibody (27106-1-AP)

    IF Staining of HeLa using 27106-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 27106-1-AP (CCDC124 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, human placenta tissue, mouse liver tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27106-1-AP is CCDC124 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC124 fusion protein Ag25930 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 124
Observed molecular weight 30-32 kDa
GenBank accession numberBC013949
Gene symbol CCDC124
Gene ID (NCBI) 115098
RRIDAB_2880758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC124 is a novel centrosomal and midbody protein that is highly conserved among species. It is ubiquitously expressed with high expression in brain, placenta, liver, spleen, and prostate. At telophase, CCDC124 dissociates from centrosomes and relocalizes to the midzone, subsequently accumulating at the midbody at cytokinesis. It interacts with the Ras-guanine nucleotide exchange factor 1B (RasGEF1B) and is involved in cytokinesis.

Protocols

Product Specific Protocols
WB protocol for CCDC124 antibody 27106-1-APDownload protocol
IF protocol for CCDC124 antibody 27106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Protein J

Live Cell Protein Imaging of Tandem Complemented-GFP11-Tagged Coiled-Coil Domain-Containing Protein-124 Identifies this Factor in G3BP1-Induced Stress-Granules

Authors - Kübra Hacibeyoğlu
View Article