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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC134 Polyclonal antibody

CCDC134 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, hamster

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26500-1-AP

Synonyms

CCDC134

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC134 Polyclonal antibody (26500-1-AP)

    WB analysis using 26500-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26500-1-AP (CCDC134 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, CHO cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26500-1-AP targets CCDC134 in WB, ELISA applications and shows reactivity with human, hamster samples.

Tested Reactivity human, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC134 fusion protein Ag21990 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 134
Calculated molecular weight 229 aa, 27 kDa
Observed molecular weight27 kda
GenBank accession numberBC017693
Gene symbol CCDC134
Gene ID (NCBI) 79879
RRIDAB_3669541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC134 (coiled-coil domain containing 134) encodes a secretory protein that can inhibit the MAPK pathway as a novel human MAPK-regulating protein (PMID: 18087676). CCDC134 is in extracellular secreted form, promotes proliferation and activation of CD8(+) T cells, suggesting a cytokine-like function (PubMed: 25125657). CCDC134 is widely expressed in normal adult tissues, tumor tissues and cell lines with the molecular weight of 27 kDa.

Protocols

Product Specific Protocols
WB protocol for CCDC134 antibody 26500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols