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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC134 Polyclonal antibody

CCDC134 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, hamster

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26500-1-AP

Synonyms

CCDC134

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC134 Polyclonal antibody (26500-1-AP)

    WB analysis using 26500-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26500-1-AP (CCDC134 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, CHO cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26500-1-AP targets CCDC134 in WB, ELISA applications and shows reactivity with human, hamster samples.

Tested Reactivity human, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC134 fusion protein Ag21990 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 134
Calculated molecular weight 229 aa, 27 kDa
Observed molecular weight27 kda
GenBank accession numberBC017693
Gene symbol CCDC134
Gene ID (NCBI) 79879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC134 (coiled-coil domain containing 134) encodes a secretory protein that can inhibit the MAPK pathway as a novel human MAPK-regulating protein (PMID: 18087676). CCDC134 is in extracellular secreted form, promotes proliferation and activation of CD8(+) T cells, suggesting a cytokine-like function (PubMed: 25125657). CCDC134 is widely expressed in normal adult tissues, tumor tissues and cell lines with the molecular weight of 27 kDa.

Protocols

Product Specific Protocols
WB protocol for CCDC134 antibody 26500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols