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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC158 Polyclonal antibody

CCDC158 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 20396-1-AP

Synonyms

CCDC158

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using CCDC158 Polyclonal antibody (20396-1-AP)

    WB analysis of mouse testis using 20396-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20396-1-AP (CCDC158 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using CCDC158 Polyclonal antibody (20396-1-AP)

    WB analysis of human testis using 20396-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20396-1-AP (CCDC158 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CCDC158 Polyclonal antibody (20396-1-AP)

    IP experiment of mouse testis using 20396-1-AP

    IP result of anti-CCDC158 (IP:20396-1-AP, 4ug; Detection:20396-1-AP 1:500) with mouse testis tissue lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CCDC158 Polyclonal antibody (20396-1-AP)

    IHC staining of human kidney using 20396-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CCDC158 Polyclonal antibody (20396-1-AP)

    IHC staining of human kidney using 20396-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC158 Polyclonal antibody (20396-1-AP)

    IHC staining of human testis using 20396-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC158 Polyclonal antibody (20396-1-AP)

    IHC staining of human testis using 20396-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20396-1-AP targets CCDC158 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC158 fusion protein Ag14049 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 158
Calculated molecular weight 1113 aa, 127 kDa
Observed molecular weight 127-140 kDa
GenBank accession numberBC035224
Gene symbol CCDC158
Gene ID (NCBI) 339965
RRIDAB_10694278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCDC158 antibody 20396-1-APDownload protocol
IHC protocol for CCDC158 antibody 20396-1-APDownload protocol
IP protocol for CCDC158 antibody 20396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

J Cell Physiol

CCDC158: A novel regulator in renal proximal tubular endocytosis unveiled through exome sequencing and interactome analysis

Authors - Tjessa Bondue
View Article