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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC18 Polyclonal antibody

CCDC18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30024-1-AP

Synonyms

CCDC18, dJ717I23.1, NY SAR 41, RP4 717I23.1, Sarcoma antigen NY SAR 24

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC18 Polyclonal antibody (30024-1-AP)

    WB analysis using 30024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30024-1-AP (CCDC18 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat colon tissue using CCDC18 Polyclonal antibody (30024-1-AP)

    IHC staining of rat colon using 30024-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 30024-1-AP (CCDC18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using CCDC18 Polyclonal antibody (30024-1-AP)

    IHC staining of rat colon using 30024-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 30024-1-AP (CCDC18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CCDC18 Polyclonal antibody (30024-1-AP)

    IF Staining of U2OS using 30024-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCDC18 antibody (30024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, Daudi cells, HL-60 cells, HeLa cells
Positive IHC detected inrat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30024-1-AP targets CCDC18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC18 fusion protein Ag32681 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 18
Observed molecular weight151 kDa
GenBank accession numberBC156745
Gene symbol CCDC18
Gene ID (NCBI) 343099
RRIDAB_2935502
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC18 (Coiled-coil domain-containing protein 18) is a Protein that in humans is encoded by the CCDC18 gene. CCDC18 has 2 isoforms with the MW of 168 kDa and 151 kDa.

Protocols

Product Specific Protocols
WB protocol for CCDC18 antibody 30024-1-APDownload protocol
IHC protocol for CCDC18 antibody 30024-1-APDownload protocol
IF protocol for CCDC18 antibody 30024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols