Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
Product Information
21209-1-AP targets CCDC25 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CCDC25 fusion protein Ag15593 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 25 |
| Calculated molecular weight | 208 aa, 24 kDa |
| Observed molecular weight | 24-26 kDa |
| GenBank accession number | BC032588 |
| Gene Symbol | CCDC25 |
| Gene ID (NCBI) | 55246 |
| RRID | AB_10732690 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86WR0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC25 antibody 21209-1-AP | Download protocol |
| IHC protocol for CCDC25 antibody 21209-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.
| ||
Nat Cell Biol Eosinophil extracellular traps drive asthma progression through neuro-immune signals.
| ||
ACS Nano Choline Phosphate-Grafted Nanozymes as Universal Extracellular Vesicle Probes for Bladder Cancer Detection | ||
FASEB J Coiled-coil domain containing 25 (CCDC25) regulates cell proliferation, migration, and invasion in clear cell renal cell carcinoma by targeting the ILK-NF-κB signaling pathway | ||
J Control Release Cell membrane derived liposomes loaded with DNase I target neutrophil extracellular traps which inhibits colorectal cancer liver metastases |