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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC25 Recombinant antibody (84484-3-RR)

    WB analysis using 84484-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84484-3-RR (CCDC25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84484-3-RR

    Affinity and Kinetic Characterization of 84484-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84484-3-RR against Human CCDC25 were performed. The affinity constant is 0.771 nM.

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CCDC25 Recombinant antibody

CCDC25 Recombinant Antibody for WB, ELISA
Cat No. 84484-3-RR
Clone No.241878H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Coiled-coil domain-containing protein 25, 241878H5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, A2780 cells, K-562 cells, MCF-7 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84484-3-RR targets CCDC25 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CCDC25 fusion protein Ag15593 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 25
Calculated molecular weight 208 aa, 24 kDa
Observed molecular weight 24-26 kDa
GenBank accession numberBC032588
Gene Symbol CCDC25
Gene ID (NCBI) 55246
RRIDAB_3672001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86WR0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCDC25 antibody 84484-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols