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- KD/KO Validated
TACO1/CCDC44 Polyclonal antibody
TACO1/CCDC44 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21147-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, mouse heart, rat heart |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 21147-1-AP is TACO1/CCDC44 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TACO1/CCDC44 fusion protein Ag15688 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 44 |
| Calculated molecular weight | 297 aa, 32 kDa |
| Observed molecular weight | 30-32 kDa |
| GenBank accession number | BC007744 |
| Gene symbol | TACO1 |
| Gene ID (NCBI) | 51204 |
| RRID | AB_10732604 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TACO1 (translational activator of cytochrome oxidase subunit I) is required for COXI translation. Mutations in TACO1 cause Leigh syndrome (LS) and cytochrome c oxidase deficiency. (27319982)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TACO1/CCDC44 antibody 21147-1-AP | Download protocol |
| IHC protocol for TACO1/CCDC44 antibody 21147-1-AP | Download protocol |
| IP protocol for TACO1/CCDC44 antibody 21147-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. | ||
Nat Commun Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.
| ||
EMBO J Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation. | ||
Free Radic Biol Med PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis | ||
Biochem Biophys Res Commun Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice. |
