- Featured Product
- KD/KO Validated
CCDC50 Polyclonal antibody
CCDC50 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 21082-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, L02 cells, mouse lung tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human kidney tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
21082-1-AP targets CCDC50 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CCDC50 fusion protein Ag15293 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 50 |
| Calculated molecular weight | 482 aa, 56 kDa |
| Observed molecular weight | 36-38 kDa |
| GenBank accession number | BC065004 |
| Gene symbol | CCDC50 |
| Gene ID (NCBI) | 152137 |
| RRID | AB_10695770 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC50 antibody 21082-1-AP | Download protocol |
| IHC protocol for CCDC50 antibody 21082-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep CCDC50 promotes tumor growth through regulation of lysosome homeostasis
| ||
EMBO Rep CCDC50 suppresses NLRP3 inflammasome activity by mediating autophagic degradation of NLRP3
| ||
Autophagy CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity |
