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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC50 Monoclonal antibody (68369-1-Ig)

    WB analysis using 68369-1-Ig (same clone as 68369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68369-1-Ig (CCDC50 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using CCDC50 Monoclonal antibody (68369-1-Ig)

    FC experiment of A549 using 68369-1-Ig (same clone as 68369-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug CCDC50 Monoclonal antibody (68369-1-Ig, Clone:2F12G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.

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CCDC50 Monoclonal antibody, PBS Only

CCDC50 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 68369-1-PBS
Clone No.2F12G7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Synonyms

YMER, Protein Ymer, DFNA44, Coiled-coil domain-containing protein 50, C3orf6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68369-1-PBS targets CCDC50 in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCDC50 fusion protein Ag15889 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 50
Calculated molecular weight 482 aa, 56 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberBC065004
Gene Symbol CCDC50
Gene ID (NCBI) 152137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IVM0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326)