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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC56/COA3 Polyclonal antibody

CCDC56/COA3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20427-1-AP

Synonyms

CCDC56, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 12 kDa, HSPC009, Cytochrome c oxidase assembly factor 3 homolog, mitochondrial, Coiled-coil domain-containing protein 56

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCDC56/COA3 Polyclonal antibody (20427-1-AP)

    IHC staining of human colon cancer using 20427-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCDC56/COA3 Polyclonal antibody (20427-1-AP)

    IHC staining of human colon cancer using 20427-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCDC56/COA3 Polyclonal antibody (20427-1-AP)

    IHC staining of human liver cancer using 20427-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCDC56/COA3 Polyclonal antibody (20427-1-AP)

    IHC staining of human liver cancer using 20427-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CCDC56/COA3 Polyclonal antibody (20427-1-AP)

    IF Staining of U2OS using 20427-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCDC56 antibody (20427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CCDC56/COA3 Polyclonal antibody (20427-1-AP)

    IF Staining of A431 using 20427-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC56 antibody (20427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, U2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

20427-1-AP targets CCDC56/COA3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC56/COA3 fusion protein Ag14260 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 56
Calculated molecular weight 106 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC002698
Gene symbol CCDC56
Gene ID (NCBI) 28958
RRIDAB_10695182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human COX assembly factor 3 (COA3), also known as CCDC56 (coiled-coil domain-containing protein 56), is a mitochondrial transmembrane protein responsible for cytochrome c oxidase (COX) protein complex assembly. CCDC56/COA3 is located on chromosome 17 (17q.21.31) and encodes an 11.7-kDa protein with predicted transmembrane and coiled-coil domains (PMID: 22610097). CCDC56/COA3 is overexpressed at both mRNA and protein levels in human NSCLC cells, mainly as a result of decreased miR-338-3p level (PMID: 36119836).

Protocols

Product Specific Protocols
IHC protocol for CCDC56/COA3 antibody 20427-1-APDownload protocol
IF protocol for CCDC56/COA3 antibody 20427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Cancer Res

COA3 overexpression promotes non-small cell lung cancer metastasis by reprogramming glucose metabolism

Authors - Hongwei Lin
View Article