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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC65 Polyclonal antibody

CCDC65 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 24376-1-AP

Synonyms

Testis development protein NYD-SP28, NYD SP28, Dynein regulatory complex subunit 2, DRC2, Coiled-coil domain-containing protein 65

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using CCDC65 Polyclonal antibody (24376-1-AP)

    WB analysis of mouse testis using 24376-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24376-1-AP (CCDC65 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using CCDC65 Polyclonal antibody (24376-1-AP)

    WB analysis of rat testis using 24376-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 24376-1-AP (CCDC65 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CCDC65 Polyclonal antibody (24376-1-AP)

    IP experiment of mouse testis using 24376-1-AP

    IP result of anti-CCDC65 (IP:24376-1-AP, 4ug; Detection:24376-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24376-1-AP targets CCDC65 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC65 fusion protein Ag19357 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 65
Calculated molecular weight 484 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC039317
Gene symbol CCDC65
Gene ID (NCBI) 85478
RRIDAB_2879513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC65, also known as DRC2, is a sperm tail protein highly expressed in adult testis, spermatocytes, and spermatids. It is a component of the nexin-dynein regulatory complex (N-DRC), a structure important for regulating and orchestrating dynein activity. Mutations in CCDC65 are associated with primary ciliary dyskinesia (PCD), a genetic disorder that affects the structure and function of cilia(PMID: 23991085).

Protocols

Product Specific Protocols
WB protocol for CCDC65 antibody 24376-1-APDownload protocol
IP protocol for CCDC65 antibody 24376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

CCDC65, a Gene Knockout that leads to Early Death of Mice, acts as a potentially Novel Tumor Suppressor in Lung Adenocarcinoma.

Authors - Ziyan Zhang
View Article