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  • Featured Product
  • KD/KO Validated

CCDC94 Polyclonal antibody

CCDC94 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19431-1-AP

Synonyms

CCDC94

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CCDC94 Polyclonal antibody (19431-1-AP)

    WB analysis of mouse brain using 19431-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19431-1-AP (CCDC94 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CCDC94 Polyclonal antibody (19431-1-AP)

    WB analysis of HeLa using 19431-1-AP

    WB result of CCDC94 antibody (19431-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC94 transfected HeLa cells.

  • Western Blot (WB) analysis of mouse brain tissue using CCDC94 Polyclonal antibody (19431-1-AP)

    WB analysis of mouse brain using 19431-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19431-1-AP (CCDC94 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CCDC94 Polyclonal antibody (19431-1-AP)

    IHC staining of human placenta using 19431-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19431-1-AP (CCDC94 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CCDC94 Polyclonal antibody (19431-1-AP)

    IHC staining of human placenta using 19431-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19431-1-AP (CCDC94 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19431-1-AP targets CCDC94 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC94 fusion protein Ag13764 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 94
Calculated molecular weight 323 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC000561
Gene symbol CCDC94
Gene ID (NCBI) 55702
RRIDAB_10641994
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

Protocols

Product Specific Protocols
WB protocol for CCDC94 antibody 19431-1-APDownload protocol
IHC protocol for CCDC94 antibody 19431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols