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CCDC94 Monoclonal antibody

CCDC94 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1G9

Cat no : 68324-1-Ig

Synonyms

YJU2, Coiled-coil domain-containing protein 94, 1D1G9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CCDC94 Monoclonal antibody (68324-1-Ig)

    WB analysis of HeLa using 68324-1-Ig

    WB result of CCDC94 antibody (68324-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC94 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CCDC94 Monoclonal antibody (68324-1-Ig)

    WB analysis using 68324-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68324-1-Ig (CCDC94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CCDC94 Monoclonal antibody (68324-1-Ig)

    IF Staining of A431 using 68324-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC94 antibody (68324-1-Ig, Clone: 1D1G9 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68324-1-Ig targets CCDC94 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCDC94 fusion protein Ag13772 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 94
Calculated molecular weight 323 aa, 37 kDa
Observed molecular weight40 kDa
GenBank accession numberBC000561
Gene symbol CCDC94
Gene ID (NCBI) 55702
RRIDAB_2935396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

Protocols

Product Specific Protocols
WB protocol for CCDC94 antibody 68324-1-IgDownload protocol
IF protocol for CCDC94 antibody 68324-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols