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CCDC94 Monoclonal antibody

CCDC94 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1G9

Cat no : 68324-1-Ig

Synonyms

YJU2, Coiled-coil domain-containing protein 94, 1D1G9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CCDC94 Monoclonal antibody (68324-1-Ig)

    WB analysis of HeLa using 68324-1-Ig

    WB result of CCDC94 antibody (68324-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC94 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CCDC94 Monoclonal antibody (68324-1-Ig)

    WB analysis using 68324-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68324-1-Ig (CCDC94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CCDC94 Monoclonal antibody (68324-1-Ig)

    IF Staining of A431 using 68324-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC94 antibody (68324-1-Ig, Clone: 1D1G9 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68324-1-Ig targets CCDC94 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCDC94 fusion protein Ag13772 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 94
Calculated molecular weight 323 aa, 37 kDa
Observed molecular weight40 kDa
GenBank accession numberBC000561
Gene symbol CCDC94
Gene ID (NCBI) 55702
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

Protocols

Product Specific Protocols
WB protocol for CCDC94 antibody 68324-1-IgDownload protocol
IF protocol for CCDC94 antibody 68324-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols