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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC94 Recombinant antibody

CCDC94 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240517E9

Cat no : 83580-5-RR

Synonyms

YJU2, Coiled-coil domain-containing protein 94, 240517E9

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CCDC94 Recombinant antibody (83580-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83580-5-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83580-5-RR (CCDC94 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CCDC94 Recombinant antibody (83580-5-RR)

    IF Staining of A549 using 83580-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-5-RR, Clone: 240517E9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using CCDC94 Recombinant antibody (83580-5-RR)

    FC experiment of A431 using 83580-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CCDC94 Recombinant antibody (83580-5-RR, Clone:240517E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83580-5-RR

    Affinity and Kinetic Characterization of 83580-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83580-5-RR against Human CCDC94 were performed. The affinity constant is 1.02 nM.

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Tested Applications

Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83580-5-RR is CCDC94 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CCDC94 fusion protein Ag13764 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 94
Calculated molecular weight 323 aa, 37 kDa
GenBank accession numberBC000561
Gene symbol CCDC94
Gene ID (NCBI) 55702
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

Protocols

Product Specific Protocols
IHC protocol for CCDC94 antibody 83580-5-RRDownload protocol
IF protocol for CCDC94 antibody 83580-5-RRDownload protocol
FC protocol for CCDC94 antibody 83580-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols