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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CCDC99 Polyclonal antibody

CCDC99 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 24689-1-AP

Synonyms

CCDC99, hSpindly, MU-RMS-40.4A, Protein Spindly, SPDL1, spdly

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC99 Polyclonal antibody (24689-1-AP)

    WB analysis using 24689-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24689-1-AP (CCDC99 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CCDC99 Polyclonal antibody (24689-1-AP)

    WB analysis of HeLa using 24689-1-AP

    WB result of CCDC99 antibody (24689-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC99 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CCDC99 Polyclonal antibody (24689-1-AP)

    IP experiment of HEK-293 using 24689-1-AP

    IP result of anti-CCDC99 (IP:24689-1-AP, 4ug; Detection:24689-1-AP 1:2000) with HEK-293 cells lysate 1400 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCDC99 Polyclonal antibody (24689-1-AP)

    IHC staining of human liver cancer using 24689-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCDC99 Polyclonal antibody (24689-1-AP)

    IHC staining of human liver cancer using 24689-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HEK293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24689-1-AP targets CCDC99 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC99 fusion protein Ag20430 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 99
Calculated molecular weight 605 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC012568
Gene symbol CCDC99
Gene ID (NCBI) 54908
RRIDAB_2879673
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCDC99 antibody 24689-1-APDownload protocol
IHC protocol for CCDC99 antibody 24689-1-APDownload protocol
IP protocol for CCDC99 antibody 24689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Cells

Identification of Ku70 Domain-Specific Interactors Using BioID2.

Authors - Sanna Abbasi
View Article
humanWB

PLoS One

Inhibiting the expression of spindle appendix cooled coil protein 1 can suppress tumor cell growth and metastasis and is associated with cancer immune cells in esophageal squamous cell carcinoma

Authors - Tao Liu
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Martijn (Verified Customer) (03-02-2023)

Antibody seems to work really well for western blotting, the signal is strong, and it doesn't show a lot of background. For immunofluorescence it does not give a clear signal, and it looks like it only shows background

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: WB: 1:1000, IF: 1:100
  • Cell Tissue Type: HeLa
CCDC99 Antibody Western Blot,Immunofluorescence validation (WB: 1:1000, IF: 1:100 dilution) in HeLa (Cat no:24689-1-AP)