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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCHCR1 Polyclonal antibody

CCHCR1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21869-1-AP

Synonyms

Putative gene 8 protein, Pg8, HCR, Coiled-coil alpha-helical rod protein 1, C6orf18

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using CCHCR1 Polyclonal antibody (21869-1-AP)

    IHC staining of human colon using 21869-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21869-1-AP (CCHCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CCHCR1 Polyclonal antibody (21869-1-AP)

    IHC staining of human colon using 21869-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21869-1-AP (CCHCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CCHCR1 Polyclonal antibody (21869-1-AP)

    IF Staining of HEK-293 using 21869-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21869-1-AP (CCHCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21869-1-AP targets CCHCR1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCHCR1 fusion protein Ag16141 相同性解析による交差性が予測される生物種
Full Name coiled-coil alpha-helical rod protein 1
Calculated molecular weight 871 aa, 99 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC110534
Gene symbol CCHCR1
Gene ID (NCBI) 54535
RRIDAB_2878932
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCHCR1, also named as C6orf18 or Putative gene 8 protein, is a 782 amino acid protein, which is a nuclear protein abundantly expressed in heart, liver, skeletal muscle, kidney and pancreas, and to a lesser extent in lung and placenta. HCR is overexpressed in keratinocytes of psoriatic lesions. HCR is associated with susceptibility to psoriasis, a chronic inflammatory dermatosis that affects approximately 2% of the population. CCHCR1 may be a regulator of keratinocyte proliferation or differentiation.

Protocols

Product Specific Protocols
IHC protocol for CCHCR1 antibody 21869-1-APDownload protocol
IF protocol for CCHCR1 antibody 21869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols