Validation Data Gallery
Tested Applications
| Positive WB detected in | SGC-7901 cells, mouse stomach tissue, rat stomach tissue, mouse pancreas tissue, rat pancreas tissue, mouse bladder tissue, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85205-1-RR targets CCKAR-specific in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | cholecystokinin A receptor |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | NM_000730 |
| Gene Symbol | CCKAR |
| Gene ID (NCBI) | 886 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P32238 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cholecystokinin (CCK) receptor, including CCKAR and CCKBR, is a receptor for cholecystokinin, mainly regulating pancreatic growth and enzyme secretion, and smooth muscle contraction of the gallbladder and stomach (PMID: 34556863). CCKAR (Cholecystokinin receptor type A) is primarily expressed in the alimentary tract, while CCKBR is mainly found in the brain and the stomach (PMID: 16816139). CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer (PMID: 36733361). The calculated MW of CCKAR is 48 kDa, but the observed MW is around 75 kDa, which may be with glycosylation modification.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCKAR-specific antibody 85205-1-RR | Download protocol |
| IP protocol for CCKAR-specific antibody 85205-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |