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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using CCKBR-specific Polyclonal antibody (16549-1-AP)

    WB analysis of BxPC-3 using 16549-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using CCKBR-specific Polyclonal antibody (16549-1-AP)

    WB analysis of SGC-7901 using 16549-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using CCKBR-specific Polyclonal antibody (16549-1-AP)

    WB analysis of human stomach using 16549-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CCKBR-specific Polyclonal antibody (16549-1-AP)

    IF Staining of BxPC-3 using 16549-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 16549-1-AP (CCKBR-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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CCKBR-specific Polyclonal antibody

CCKBR-specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16549-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CCKBR, CCK2-R, CCK2R, CCK2 R, CCK BR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, human stomach tissue, SGC-7901 cells
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16549-1-AP targets CCKBR-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cholecystokinin B receptor
Calculated molecular weight 56 kDa
Observed molecular weight 64 kDa, 50 kDa
GenBank accession numberNM_176875
Gene Symbol CCKBR
Gene ID (NCBI) 887
RRIDAB_2070508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32239
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cholecystokinin B receptor (CCKBR) is a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.

Protocols

Product Specific Protocols
WB protocol for CCKBR-specific antibody 16549-1-APDownload protocol
IF protocol for CCKBR-specific antibody 16549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Pharm

Pseudomonas Exotoxin A-Based Immunotherapy Targeting CCK2R-Expressing Colorectal Malignancies: An In Vitro and In Vivo Evaluation.

Authors - Jiang Chang
View Article
humanIF

J Cancer Res Clin Oncol

Cholecystokinin type 2 receptor in colorectal cancer: diagnostic and therapeutic target.

Authors - Jiang Chang
View Article