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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCL17 Polyclonal antibody

CCL17 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 22342-1-AP

Synonyms

A 152E5.3, ABCD 2, C C motif chemokine 17, CC chemokine TARC, CCL17, SCYA17, Small inducible cytokine A17, TARC

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human colon tissue using CCL17 Polyclonal antibody (22342-1-AP)

    IHC staining of human colon using 22342-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CCL17 Polyclonal antibody (22342-1-AP)

    IHC staining of human colon using 22342-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CCL17 Polyclonal antibody (22342-1-AP)

    IHC staining of human placenta using 22342-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CCL17 Polyclonal antibody (22342-1-AP)

    IHC staining of human placenta using 22342-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCL17 Polyclonal antibody (22342-1-AP)

    IHC staining of human colon cancer using 22342-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCL17 Polyclonal antibody (22342-1-AP)

    IHC staining of human colon cancer using 22342-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman colon tissue, human placenta tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

The immunogen of 22342-1-AP is CCL17 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCL17 fusion protein Ag17832 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 17
Calculated molecular weight 94 aa, 11 kDa
GenBank accession numberBC112066
Gene symbol CCL17
Gene ID (NCBI) 6361
RRIDAB_2879081
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCL17, also named as TARC, is a member of the CC chemokine family, and is highly expressed by thymus and other cells, including keratinocytes, endothelial cells, dendritic cells, bronchial epithelial cells and fibroblasts. CCL17 plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. CCL17 induced chemotaxis and Ca2+ influx in the cells stably expressing CCR4, thereby demonstrating the ligand-receptor relationship between CCL17 and CCR4.

Protocols

Product Specific Protocols
IHC protocol for CCL17 antibody 22342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Br J Haematol

Expression of multiple cytokines and chemokine receptor 4 in angioimmunoblastic T-cell lymphoma with abundant plasma cell infiltration involving the skin.

Authors - Shoko Nakayama
View Article
humanIHC

Hemasphere

NOTCH1 fusions in pediatric T-cell lymphoblastic lymphoma: A high-risk subgroup with CCL17 (TARC) levels as diagnostic biomarker

Authors - Emma Kroeze
View Article