• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCL27 Polyclonal antibody

CCL27 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22301-1-AP

Synonyms

ALP, C C motif chemokine 27, CC chemokine ILC, CCL27, CTACK, CTAK, ESKINE, IL 11 R alpha locus chemokine, ILC, PESKY, SCYA27, Skinkine, Small inducible cytokine A27

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human testis tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human testis using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human testis using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human skin using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human skin using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human skin cancer using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human skin cancer using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human liver using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human liver using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human skin using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human skin using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human skin using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human skin using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human liver cancer using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCL27 Polyclonal antibody (22301-1-AP)

    IHC staining of human liver cancer using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CCL27 Polyclonal antibody (22301-1-AP)

    IF Staining of A431 using 22301-1-AP

    Immunofluorescent analysis of A431 cells using 22301-1-AP (CCL27 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CCL27 Polyclonal antibody (22301-1-AP)

    IF Staining of A431 using 22301-1-AP

    Immunofluorescent analysis of A431 cells using 22301-1-AP (CCL27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive IHC detected inhuman testis tissue, human liver tissue, human liver cancer tissue, human skin tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22301-1-AP targets CCL27 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 27
Calculated molecular weight 13 kDa
GenBank accession numberNM_006664
Gene symbol CCL27
Gene ID (NCBI) 10850
RRIDAB_2879065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCL27, also known as CTACK, is a member of the CC chemokine subfamily. CCL27 is predominantly expressed in skin and selectively attracts cutaneous lymphocyte antigen-positive T cells and Langerhans cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. CCL27 is a ligand to CCR10. The binding of CCL27 to CCR10 on T cells promotes their migration to the skin, where they can then participate in local immune responses.

Protocols

Product Specific Protocols
IHC protocol for CCL27 antibody 22301-1-APDownload protocol
IF protocol for CCL27 antibody 22301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols