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Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using CCL28 Polyclonal antibody (18214-1-AP)

    WB analysis of human spleen using 18214-1-AP (same clone as 18214-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CCL28 Polyclonal antibody (18214-1-AP)

    WB analysis of Jurkat using 18214-1-AP (same clone as 18214-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using CCL28 Polyclonal antibody (18214-1-AP)

    WB analysis of PC-3 using 18214-1-AP (same clone as 18214-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using CCL28 Polyclonal antibody (18214-1-AP)

    WB analysis of mouse spleen using 18214-1-AP (same clone as 18214-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCL28 Polyclonal antibody (18214-1-AP)

    IHC staining of human colon cancer using 18214-1-AP (same clone as 18214-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18214-1-AP (CCL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCL28 Polyclonal antibody (18214-1-AP)

    IHC staining of human colon cancer using 18214-1-AP (same clone as 18214-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18214-1-AP (CCL28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

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CCL28 Polyclonal antibody, PBS Only

CCL28 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18214-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18214-1-PBS targets CCL28 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCL28 fusion protein Ag12920 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 28
Calculated molecular weight 127 aa, 14 kDa
Observed molecular weight 8 kDa-9 kDa
GenBank accession numberBC069532
Gene Symbol CCL28
Gene ID (NCBI) 56477
RRIDAB_2262251
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRJ3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCL28, also named as SCYA28, CCK1 and MEC, belongs to the intercrine beta (chemokine CC) family. It has chemotactic activity for resting CD4, CD8 T-cells and eosinophils. CCL28 binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. CCL28 is expressed in a variety of human and mouse tissues, and it appears to be predominantly produced by epithelial cells. It is produced by epithelial cells of these tissues suggesting that this chemokine can play an important role by linking homing mechanisms between the gut, nasal mucosa and mammary gland (MG). In WB test, CCL28 is 14kd and 8-9kd(a putatively to a degradation fragment).