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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCL3L1 Polyclonal antibody (12859-1-AP)

    WB analysis using 12859-1-AP

    PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 12859-1-AP (CCL3L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CCL3L1 Polyclonal antibody

CCL3L1 Polyclonal Antibody for WB, ELISA
Cat No. 12859-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

464.2, C C motif chemokine 3 like 1, CCL3L1, CCL3L3, D17S1718, G0S19 2, LD78, LD78 beta(1 70), LD78BETA, MIP1AP, PAT 464.2, SCYA3L, SCYA3L1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12859-1-AP targets CCL3L1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCL3L1 fusion protein Ag3891 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 3-like 1
Calculated molecular weight 10 kDa
Observed molecular weight10 kDa
GenBank accession numberBC027888
Gene Symbol CCL3L1
Gene ID (NCBI) 6349
RRIDAB_3085401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16619
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCL3L1 is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this gene binds to several chemokine receptors, including chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. The copy number of this gene varies among individuals, where most individuals have one to six copies, and a minority of individuals have zero or more than six copies. There are conflicting reports about copy number variation of this gene and its correlation to disease susceptibility.

Protocols

Product Specific Protocols
WB protocol for CCL3L1 antibody 12859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols