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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CCM2 Polyclonal antibody

CCM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26270-1-AP

Synonyms

C7orf22, CCM2, Malcavernin, OSM, PP10187

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CCM2 Polyclonal antibody (26270-1-AP)

    WB analysis of MCF-7 using 26270-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26270-1-AP (CCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCM2 Polyclonal antibody (26270-1-AP)

    IHC staining of human liver cancer using 26270-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26270-1-AP (CCM2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCM2 Polyclonal antibody (26270-1-AP)

    IHC staining of human liver cancer using 26270-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26270-1-AP (CCM2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCM2 Polyclonal antibody (26270-1-AP)

    IF Staining of MCF-7 using 26270-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 26270-1-AP (CCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26270-1-AP is CCM2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCM2 fusion protein Ag24233 相同性解析による交差性が予測される生物種
Full Name cerebral cavernous malformation 2
Calculated molecular weight 49 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC004903
Gene symbol CCM2
Gene ID (NCBI) 83605
RRIDAB_2880454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCM2 antibody 26270-1-APDownload protocol
IHC protocol for CCM2 antibody 26270-1-APDownload protocol
IF protocol for CCM2 antibody 26270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Endocrinology

Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice

Authors - Abraham Behrmann
  • KD Validated
View Article
humanWB

Biochem J

CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading.

Authors - Koichi Miura
  • KD Validated
View Article