Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 18202-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18202-1-AP
WB result of Cyclin A2 antibody (18202-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells.
WB analysis using 18202-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 18202-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18202-1-AP (CCNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 18202-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 18202-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 18202-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HL-60 using 18202-1-AP
IP result of anti-Cyclin A2 (IP:18202-1-AP, 3ug; Detection:18202-1-AP 1:500) with HL-60 cells lysate 4000ug.
IP experiment of HeLa using 18202-1-AP
IP result of anti-Cyclin A2 (IP:18202-1-AP, 4ug; Detection:18202-1-AP 1:20000) with HeLa cells lysate 1320 ug.
IHC staining of human tonsillitis using 18202-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 18202-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 18202-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 18202-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 18202-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 18202-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 18202-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin A2 antibody (18202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of MCF-7 using 18202-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin A2 antibody (18202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 18202-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclin A2 (18202-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Featured Product
KD/KO Validated

Cyclin A2 Polyclonal antibody

Cyclin A2 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Cat No. 18202-1-AP

Publications(186)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, FC (Intra), IP, CoIP, ELISA

Synonyms

CCNA2, CyclinA2, Cyclin-A, Cyclin A, CCNA

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (186)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, K-562 cells, U-937 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected in	HeLa cells, HL-60 cells
Positive IHC detected in	human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, MCF-7 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 178 publications below
CoIP	See 1 publications below

Product Information

18202-1-AP targets Cyclin A2 in WB, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cyclin A2 fusion protein Ag12765 相同性解析による交差性が予測される生物種
Full Name	cyclin A2
Calculated molecular weight	432 aa, 49 kDa
Observed molecular weight	48-52 kDa
GenBank accession number	BC104783
Gene Symbol	Cyclin A2
Gene ID (NCBI)	890
RRID	AB_10597084
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20248
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.

Protocols

Product Specific Protocols
WB protocol for Cyclin A2 antibody 18202-1-AP	Download protocol
IHC protocol for Cyclin A2 antibody 18202-1-AP	Download protocol
IF protocol for Cyclin A2 antibody 18202-1-AP	Download protocol
IP protocol for Cyclin A2 antibody 18202-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Cell Metab Sphingolipid metabolism controls mammalian heart regeneration Authors - Xiaoqian Ji View Article
mouse	WB	Nat Metab LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration Authors - Yalan Deng View Article
		Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis Authors - Kaiwen Bao View Article
human	WB	Mol Cancer CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA. Authors - Bing Yao View Article
mouse		Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. Authors - Weimin Yu View Article
human	WB	Sci Adv The p23 co-chaperone is a succinate-activated COX-2 transcription factor in lung adenocarcinoma tumorigenesis Authors - Zhenlong Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18202-1-AP

WB analysis of HeLa using 18202-1-AP

WB analysis using 18202-1-AP

WB analysis of NIH/3T3 using 18202-1-AP

WB analysis of K-562 using 18202-1-AP

WB analysis of U-937 using 18202-1-AP

WB analysis of MCF-7 using 18202-1-AP

IP experiment of HL-60 using 18202-1-AP

IP experiment of HeLa using 18202-1-AP

IHC staining of human tonsillitis using 18202-1-AP

IHC staining of human tonsillitis using 18202-1-AP

IHC staining of human colon cancer using 18202-1-AP

IHC staining of human colon cancer using 18202-1-AP

IHC staining of human breast cancer using 18202-1-AP

IHC staining of human breast cancer using 18202-1-AP

IF Staining of HepG2 using 18202-1-AP

IF Staining of MCF-7 using 18202-1-AP

FC experiment of HeLa using 18202-1-AP

Cyclin A2 Polyclonal antibody

Cyclin A2 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Sphingolipid metabolism controls mammalian heart regeneration

LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration

A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

The p23 co-chaperone is a succinate-activated COX-2 transcription factor in lung adenocarcinoma tumorigenesis