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KD/KO Validated

Cyclin B2 Polyclonal antibody

Name: Cyclin B2 Polyclonal antibody
Brand: Proteintech
SKU: 21644-1-AP

Cyclin B2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21644-1-AP

Synonyms

CCNB2, cyclin B2, G2/mitotic specific cyclin B2, HsT17299

Validation Data Gallery

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AllWBIPIHC

WB analysis using 21644-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 21644-1-AP
WB result of Cyclin B2 antibody (21644-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B2 transfected A431 cells.
WB analysis of HeLa using 21644-1-AP
Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.
WB analysis of DU 145 using 21644-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 21644-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 21644-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 21644-1-AP
IP result of anti-Cyclin B2 (IP:21644-1-AP, 4ug; Detection:21644-1-AP 1:1000) with A431 cells lysate 2000ug.
IHC staining of human cervical cancer using 21644-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21644-1-AP (Cyclin B2 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human cervical cancer using 21644-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21644-1-AP (Cyclin B2 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human colon cancer using 21644-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 21644-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 21644-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 21644-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 21644-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, DU 145 cells, rat testis tissue, HeLa cells, K-562 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human cervical cancer tissue, human testis tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 19 publications below
IHC	See 5 publications below
IP	See 1 publications below

Product Information

21644-1-AP targets Cyclin B2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cyclin B2 fusion protein Ag16329 相同性解析による交差性が予測される生物種
Full Name	cyclin B2
Calculated molecular weight	398 aa, 45 kDa
Observed molecular weight	51 kDa
GenBank accession number	BC105086
Gene symbol	Cyclin B2
Gene ID (NCBI)	9133
RRID	AB_10755304
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status. Cyclin B2 can be detected double bands (PMID:1651338).

Protocols

Product Specific Protocols
WB protocol for Cyclin B2 antibody 21644-1-AP	Download protocol
IHC protocol for Cyclin B2 antibody 21644-1-AP	Download protocol
IP protocol for Cyclin B2 antibody 21644-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Phytomedicine Ganoderma lucidum polysaccharide inhibits HSC activation and liver fibrosis via targeting inflammation, apoptosis, cell cycle, and ECM-receptor interaction mediated by TGF-β/Smad signaling Authors - Chaojie Chen View Article
human	WB,IP	EMBO Rep Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint. Authors - Sikai Liu KD Validated View Article
human	WB	Oxid Med Cell Longev Therapy-resistant and -sensitive lncRNAs, SNHG1 and UBL7-AS1 promote glioblastoma cell proliferation. Authors - Mei Cao View Article
human	IHC	Elife Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation. Authors - Xiao-Tong Lin View Article
human	WB	Oncotarget The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis. Authors - Lifei Fan View Article
human	WB	Ecotoxicol Environ Saf Disruption of mitochondrial redox homeostasis as a mechanism of antimony-induced reactive oxygen species and cytotoxicity. Authors - Liling Su View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin B2 Polyclonal antibody

Cyclin B2 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 21644-1-AP

WB analysis of A431 using 21644-1-AP

WB analysis of HeLa using 21644-1-AP

WB analysis of DU 145 using 21644-1-AP

WB analysis of rat testis using 21644-1-AP

WB analysis using 21644-1-AP

IP experiment of A431 using 21644-1-AP

IHC staining of human cervical cancer using 21644-1-AP

IHC staining of human cervical cancer using 21644-1-AP

IHC staining of human colon cancer using 21644-1-AP

IHC staining of human colon cancer using 21644-1-AP

IHC staining of human testis using 21644-1-AP

IHC staining of human testis using 21644-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Ganoderma lucidum polysaccharide inhibits HSC activation and liver fibrosis via targeting inflammation, apoptosis, cell cycle, and ECM-receptor interaction mediated by TGF-β/Smad signaling

Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint.

Therapy-resistant and -sensitive lncRNAs, SNHG1 and UBL7-AS1 promote glioblastoma cell proliferation.

Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation.

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Disruption of mitochondrial redox homeostasis as a mechanism of antimony-induced reactive oxygen species and cytotoxicity.