Validation Data Gallery
Tested Applications
| Positive WB detected in | Caco-2 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, HEK-293 cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 45 publications below |
| IHC | See 1 publications below |
Product Information
10934-1-AP targets Cyclin D2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cyclin D2 fusion protein Ag1377 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin D2 |
| Calculated molecular weight | 32 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC010958 |
| Gene Symbol | Cyclin D2 |
| Gene ID (NCBI) | 894 |
| RRID | AB_2275319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin D2 antibody 10934-1-AP | Download protocol |
| IHC protocol for Cyclin D2 antibody 10934-1-AP | Download protocol |
| IF protocol for Cyclin D2 antibody 10934-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma. | ||
Cancer Cell The Oncogenic Transcription Factor RUNX1/ETO Corrupts Cell Cycle Regulation to Drive Leukemic Transformation.
| ||
Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer. | ||
Dev Cell Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells. | ||
Kidney Int Transcription factor MafB may play an important role in secondary hyperparathyroidism. | ||
Front Immunol Comprehensive single-cell and bulk transcriptomic analyses to develop an NK cell-derived gene signature for prognostic assessment and precision medicine in breast cancer |