Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
12363-1-AP targets CCNDBP1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CCNDBP1 fusion protein Ag3032 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin D-type binding-protein 1 |
| Calculated molecular weight | 360 aa, 40 kDa |
| GenBank accession number | BC009689 |
| Gene Symbol | CCNDBP1 |
| Gene ID (NCBI) | 23582 |
| RRID | AB_2877849 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95273 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCNDBP1, also named as DIP1, GCIP and HHM, is a helix-loop-helix protein which suppresses tumorigenesis. CCNDBP1 specifically interacts with one of the class III HDAC proteins, SirT6, which is important for maintaining genome stability. CCNDBP1 may negatively regulate cell cycle progression.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCNDBP1 antibody 12363-1-AP | Download protocol |
| IHC protocol for CCNDBP1 antibody 12363-1-AP | Download protocol |
| IF protocol for CCNDBP1 antibody 12363-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ. | ||
Front Oncol CCNDBP1, a Prognostic Marker Regulated by DNA Methylation, Inhibits Aggressive Behavior in Dedifferentiated Liposarcoma via Repressing Epithelial Mesenchymal Transition | ||
J Cell Mol Med LncRNA ABHD11-AS1 promotes the development of endometrial carcinoma by targeting cyclin D1. | ||
Oncol Rep Expression and prognostic significance of TRPV6 in the development and progression of pancreatic cancer. | ||
Am J Transl Res Lithium chloride inhibits cell survival, overcomes drug resistance, and triggers apoptosis in multiple myeloma via activation of the Wnt/β-catenin pathway. | ||