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Cyclin E1 Polyclonal antibody

Name: Cyclin E1 Polyclonal antibody
Brand: Proteintech
SKU: 11554-1-AP
Rating: 4.00 (2 reviews)

Cyclin E1 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Publications

304

Reviews (2)

Cat no : 11554-1-AP

Synonyms

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE

Validation Data Gallery

Filter:

AllWBIHCFC (Intra)

WB analysis using 11554-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11554-1-AP
Non-treated HeLa and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.
WB analysis using 11554-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 11554-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 11554-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11554-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 11554-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11554-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11554-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11554-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 11554-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11554-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11554-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11554-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11554-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HeLa using 11554-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclin E1 (11554-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
FC experiment of MCF-7 using 11554-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin E1 (11554-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 11554-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HT-29 cells, NIH/3T3 cells, mouse heart tissue, HeLa cells, Jurkat cells, human lung tissue, MCF-7 cells, HepG2 cells, K-562 cells, human placenta tissue
Positive IHC detected in	mouse testis tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 292 publications below
IHC	See 9 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

11554-1-AP targets Cyclin E1 in WB, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, zebrafish, artemia sinica
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cyclin E1 fusion protein Ag2110 相同性解析による交差性が予測される生物種
Full Name	cyclin E1
Calculated molecular weight	410 aa, 47 kDa
Observed molecular weight	47 kDa
GenBank accession number	BC035498
Gene symbol	Cyclin E1
Gene ID (NCBI)	898
RRID	AB_2071066
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).

Protocols

Product Specific Protocols
WB protocol for Cyclin E1 antibody 11554-1-AP	Download protocol
IHC protocol for Cyclin E1 antibody 11554-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer. Authors - Xi Jin View Article
human	WB	Adv Sci (Weinh) Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer Authors - Rong Wang View Article
mouse	IHC	Theranostics Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Authors - Meng Gao View Article
human	WB	Nat Commun Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma. Authors - Dan Su View Article
mouse	WB	Nat Commun Iron-dependent histone 3 lysine 9 demethylation controls B cell proliferation and humoral immune responses. Authors - Yuhang Jiang View Article
human	WB	EBioMedicine Shuttling SLC2A4RG is regulated by 14-3-3θ to modulate cell survival via caspase-3 and caspase-6 in human glioma. Authors - Dapeng Yun View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Tsimafei (Verified Customer) (08-04-2024)	Antibody detect a weak band around 50kDa, however the signal is weak and non-specific strong signal is present at ~75kDa Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: mESCs Cyclin E1 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:11554-1-AP)
FH Carly (Verified Customer) (11-17-2020)	Tested using EDTA plasma on an antibody microarray Applications: Other

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin E1 Polyclonal antibody

Cyclin E1 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 11554-1-AP

WB analysis of HeLa using 11554-1-AP

WB analysis using 11554-1-AP

WB analysis using 11554-1-AP

WB analysis of mouse heart using 11554-1-AP

WB analysis using 11554-1-AP

WB analysis of human lung using 11554-1-AP

WB analysis of HeLa using 11554-1-AP

WB analysis of Jurkat using 11554-1-AP

WB analysis of NIH/3T3 using 11554-1-AP

IHC staining of mouse testis using 11554-1-AP

IHC staining of human placenta using 11554-1-AP

IHC staining of human placenta using 11554-1-AP

IHC staining of human placenta using 11554-1-AP

IHC staining of human placenta using 11554-1-AP

FC experiment of HeLa using 11554-1-AP

FC experiment of MCF-7 using 11554-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction

Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.

Iron-dependent histone 3 lysine 9 demethylation controls B cell proliferation and humoral immune responses.

Shuttling SLC2A4RG is regulated by 14-3-3θ to modulate cell survival via caspase-3 and caspase-6 in human glioma.

Reviews

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