Cyclin G Polyclonal antibody

Name: Cyclin G Polyclonal antibody
Brand: Proteintech
SKU: 10897-1-AP

Cyclin G Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10897-1-AP

Synonyms

CCNG, CCNG1, CYCG1, Cyclin G, cyclin G1

Validation Data Gallery

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AllWBIHC

WB analysis of MDA-MB-453s using 10897-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10897-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10897-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 10897-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10897-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10897-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10897-1-AP (Cyclin G antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10897-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10897-1-AP (Cyclin G antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 10897-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MDA-MB-453s cells, mouse ovary tissue, HeLa cells, mouse liver tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below

Product Information

10897-1-AP targets Cyclin G in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cyclin G fusion protein Ag1157 相同性解析による交差性が予測される生物種
Full Name	cyclin G1
Calculated molecular weight	34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC007093
Gene symbol	Cyclin G
Gene ID (NCBI)	900
RRID	AB_2228613
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Cyclin G antibody 10897-1-AP	Download protocol
IHC protocol for Cyclin G antibody 10897-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev miR-488-3p Protects Cardiomyocytes against Doxorubicin-Induced Cardiotoxicity by Inhibiting CyclinG1. Authors - Mingjing Yan View Article
human	WB	Cancer Lett The C/EBPβ-LINC01133 axis promotes cell proliferation in pancreatic ductal adenocarcinoma through upregulation of CCNG1. Authors - Chen-Song Huang View Article
human	WB	J Biol Chem MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1. Authors - Fanpeng Zhao View Article
human	WB	Exp Cell Res PRMT6 functionally associates with PRMT5 to promote colorectal cancer progression through epigenetically repressing the expression of CDKN2B and CCNG1 Authors - Yuzhong Chen View Article
human	WB	Oncol Res Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2 Authors - Yusen Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin G Polyclonal antibody

Cyclin G Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of MDA-MB-453s using 10897-1-AP

WB analysis of HeLa using 10897-1-AP

WB analysis of mouse liver using 10897-1-AP

WB analysis of mouse ovary using 10897-1-AP

WB analysis of HeLa using 10897-1-AP

IHC staining of human breast cancer using 10897-1-AP

IHC staining of human breast cancer using 10897-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

miR-488-3p Protects Cardiomyocytes against Doxorubicin-Induced Cardiotoxicity by Inhibiting CyclinG1.

The C/EBPβ-LINC01133 axis promotes cell proliferation in pancreatic ductal adenocarcinoma through upregulation of CCNG1.

MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1.

PRMT6 functionally associates with PRMT5 to promote colorectal cancer progression through epigenetically repressing the expression of CDKN2B and CCNG1

Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2