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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin H Polyclonal antibody

Cyclin H Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10718-1-AP

Synonyms

CCNH, p37, p34, MO15-associated protein, MO15 associated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin H Polyclonal antibody (10718-1-AP)

    WB analysis using 10718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using Cyclin H Polyclonal antibody (10718-1-AP)

    WB analysis of HuH-7 using 10718-1-AP

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using Cyclin H Polyclonal antibody (10718-1-AP)

    WB analysis of A375 using 10718-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Cyclin H Polyclonal antibody (10718-1-AP)

    WB analysis of mouse brain using 10718-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Cyclin H Polyclonal antibody (10718-1-AP)

    IP experiment of HeLa using 10718-1-AP

    IP result of anti-Cyclin H (IP:10718-1-AP, 4ug; Detection:10718-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin H Polyclonal antibody (10718-1-AP)

    IHC staining of human breast cancer using 10718-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10718-1-AP (Cyclin H antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin H Polyclonal antibody (10718-1-AP)

    IHC staining of human breast cancer using 10718-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10718-1-AP (Cyclin H antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cyclin H Polyclonal antibody (10718-1-AP)

    IF Staining of A431 using 10718-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cyclin H antibody (10718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA431 cells, A375 cells, mouse brain tissue, HuH-7 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10718-1-AP targets Cyclin H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin H fusion protein Ag1088 相同性解析による交差性が予測される生物種
Full Name cyclin H
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC005280
Gene symbol Cyclin H
Gene ID (NCBI) 902
RRIDAB_2073749
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cyclin H antibody 10718-1-APDownload protocol
IHC protocol for Cyclin H antibody 10718-1-APDownload protocol
IF protocol for Cyclin H antibody 10718-1-APDownload protocol
IP protocol for Cyclin H antibody 10718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols