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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCNJ Polyclonal antibody (15694-1-AP)

    WB analysis using 15694-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15694-1-AP (CCNJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using CCNJ Polyclonal antibody (15694-1-AP)

    IHC staining of rat kidney using 15694-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15694-1-AP (CCNJ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CCNJ Polyclonal antibody

CCNJ Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15694-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Cyclin-J, cyclin J

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15694-1-AP targets CCNJ in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCNJ fusion protein Ag8273 相同性解析による交差性が予測される生物種
Full Name cyclin J
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC043175
Gene Symbol CCNJ
Gene ID (NCBI) 54619
RRIDAB_3085470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T5M9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCNJ (Cyclin-J) Belongs to the Cyclin family. Cyclin J is expressed in early Drosophila embryos, and it is essential for mediating intestinal epithelial cell recovery from bacterial pore-forming toxin attack in both Drosophila and mice. Upon TLR and IFN stimulation, cyclin J restrained macrophage inflammatory responses irrespective of the cell cycle control. Cyclin J interacted with CDKs and facilitated the phosphorylation of a set of CDK substrates, including FoxK1 and Drp1. Phosphorylation inhibited FoxK1-mediated expression of glycolytic genes by reducing FoxK1 nuclear localization. Cyclin J-CDK-dependent Drp1 phosphorylation also resulted in mitochondrial fission and the suppression of ROS production (PMID: 35412849).

Protocols

Product Specific Protocols
WB protocol for CCNJ antibody 15694-1-APDownload protocol
IHC protocol for CCNJ antibody 15694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols