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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCNJL Polyclonal antibody (26743-1-AP)

    WB analysis using 26743-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26743-1-AP (CCNJL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CCNJL Polyclonal antibody (26743-1-AP)

    IF Staining of HepG2 using 26743-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CCNJL antibody (26743-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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CCNJL Polyclonal antibody

CCNJL Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26743-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Cyclin-J-like protein, Cyclin J like protein, cyclin J like

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26743-1-AP targets CCNJL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCNJL fusion protein Ag25048 相同性解析による交差性が予測される生物種
Full Name cyclin J-like
Calculated molecular weight 435 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC013353
Gene Symbol CCNJL
Gene ID (NCBI) 79616
RRIDAB_3085900
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCNJL antibody 26743-1-APDownload protocol
IF protocol for CCNJL antibody 26743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols