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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCNK Polyclonal antibody

CCNK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26933-1-AP

Synonyms

CCNK, CPR4, cyclin K

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCNK Polyclonal antibody (26933-1-AP)

    WB analysis using 26933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26933-1-AP (CCNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26933-1-AP targets CCNK in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCNK fusion protein Ag25556 相同性解析による交差性が予測される生物種
Full Name cyclin K
Calculated molecular weight 64 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC015935
Gene symbol CCNK
Gene ID (NCBI) 8812
RRIDAB_2935464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin-K (CCNK) is also named as CPR4 and belongs to the cyclin family. CCNK is a regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases (PMID:10574912). It plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A) (PMID:9632813). The Cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes (PMID:22012619).

Protocols

Product Specific Protocols
WB protocol for CCNK antibody 26933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols