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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCNL1 Polyclonal antibody (13138-1-AP)

    WB analysis using 13138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CCNL1 Polyclonal antibody (13138-1-AP)

    WB analysis of COLO 320 using 13138-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CCNL1 Polyclonal antibody (13138-1-AP)

    WB analysis of PC-3 using 13138-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CCNL1 Polyclonal antibody (13138-1-AP)

    WB analysis of MCF-7 using 13138-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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CCNL1 Polyclonal antibody

CCNL1 Polyclonal Antibody for WB, ELISA
Cat No. 13138-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ania 6a, BM 001, CCNL1, Cyclin L, cyclin L1, PRO1073, UNQ530/PRO1073

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, PC-3 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13138-1-AP targets CCNL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCNL1 fusion protein Ag3731 相同性解析による交差性が予測される生物種
Full Name cyclin L1
Calculated molecular weight 526 aa, 60 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC007081
Gene Symbol CCNL1
Gene ID (NCBI) 57018
RRIDAB_10640299
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK58
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCNL1, also named as Cyclin-L1, contains a RS region and belongs to the cyclin family. CCNL1 localizes in the nucleus speckle and is Overexpressed in primary tumors of head and neck squamous cell carcinomas (HNSCC). CCNL1 as transcriptional regulator participates in regulating the pre-mRNA splicing process. It is seems to be involved in the regulation of RNA polymerase II (pol II). CCNL1 functions in association with cyclin-dependent kinases (CDKs) and has a role in the second step of splicing. It may be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC). The calculated molecular weight of CCNL1 is about 55-60 kDa, but we detected a 40-50 kDa isoform by western blotting.

Protocols

Product Specific Protocols
WB protocol for CCNL1 antibody 13138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols