Cyclin T1 Polyclonal antibody

Name: Cyclin T1 Polyclonal antibody
Brand: Proteintech
SKU: 20992-1-AP

Cyclin T1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20992-1-AP

Synonyms

CCNT1, CCNT, CYCT1, Cyclin-T1, Cyclin-T

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 20992-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 20992-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 20992-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20992-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 20992-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 20992-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 20992-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human ovary cancer using 20992-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20992-1-AP (Cyclin T1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 20992-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin T1 antibody (20992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 20992-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A431 cells, Jurkat cells, K-562 cells, Y79 cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 1 publications below

Product Information

20992-1-AP targets Cyclin T1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	cyclin T1
Calculated molecular weight	81 kDa
Observed molecular weight	81 kDa
GenBank accession number	NM_001240
Gene symbol	Cyclin T1
Gene ID (NCBI)	904
RRID	AB_10950894
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CCNT1, also named as CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.

Protocols

Product Specific Protocols
WB protocol for Cyclin T1 antibody 20992-1-AP	Download protocol
IHC protocol for Cyclin T1 antibody 20992-1-AP	Download protocol
IF protocol for Cyclin T1 antibody 20992-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Menin enhances c-Myc-mediated transcription to promote cancer progression. Authors - Gongwei Wu View Article
human	WB	Nucleic Acids Res HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters. Authors - Sebastian Eilebrecht View Article
human	WB,IF	BMC Cancer The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study Authors - Ying E View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin T1 Polyclonal antibody

Cyclin T1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 20992-1-AP

WB analysis of K-562 using 20992-1-AP

WB analysis of A431 using 20992-1-AP

WB analysis of HeLa using 20992-1-AP

WB analysis of Y79 using 20992-1-AP

WB analysis of Jurkat using 20992-1-AP

WB analysis of K-562 using 20992-1-AP

IHC staining of human ovary cancer using 20992-1-AP

IF Staining of MCF-7 using 20992-1-AP