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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using cyclin T2 Polyclonal antibody (21860-1-AP)

    WB analysis using 21860-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21860-1-AP (cyclin T2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using cyclin T2 Polyclonal antibody (21860-1-AP)

    IHC staining of mouse brain using 21860-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 4010x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using cyclin T2 Polyclonal antibody (21860-1-AP)

    IHC staining of rat brain using 21860-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using cyclin T2 Polyclonal antibody (21860-1-AP)

    IHC staining of rat brain using 21860-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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cyclin T2 Polyclonal antibody

cyclin T2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21860-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CCNT2, CycT2, Cyclin-T2, Cyclin T

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21860-1-AP targets cyclin T2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen cyclin T2 fusion protein Ag16514 相同性解析による交差性が予測される生物種
Full Name cyclin T2
Calculated molecular weight 730 aa, 81 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC114366
Gene Symbol Cyclin T2
Gene ID (NCBI) 905
RRIDAB_3085675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60583
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin T2 (CCNT2) belongs to the cyclin family. CCNT2 is regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II)  (PMID: 9499409, PMID: 15563843). Cyclin T2 is expressed at a relatively high level in freshly isolated monocytes and its level remains constant when the cells are induced to undergo macrophage differentiation (PMID: 21791050). In contrast, Cyclin T1 is expressed at low levels in monocytes and it is strongly up-regulated by a post-transcriptional mechanism when the cells are induced to differentiate to macrophages.

Protocols

Product Specific Protocols
WB protocol for cyclin T2 antibody 21860-1-APDownload protocol
IHC protocol for cyclin T2 antibody 21860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols