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  • Featured Product
  • KD/KO Validated

CCNY Monoclonal antibody, PBS Only

CCNY Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C9E3

Cat no : 66865-1-PBS

Synonyms

C10orf9, CBCP1, CCNY, CFP1, Cyc Y, Cyclin box protein 1, Cyclin fold protein 1, cyclin X, cyclin Y, FLJ95513

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCNY Monoclonal antibody (66865-1-Ig)

    WB analysis using 66865-1-Ig (same clone as 66865-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66865-1-Ig (CCNY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66865-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using CCNY Monoclonal antibody (66865-1-Ig)

    WB analysis of A549 using 66865-1-Ig (same clone as 66865-1-PBS)

    WB result of CCNY antibody (66865-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCNY transfected A549 cells. This data was developed using the same antibody clone with 66865-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CCNY Monoclonal antibody (66865-1-Ig)

    WB analysis of NIH/3T3 using 66865-1-Ig (same clone as 66865-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66865-1-Ig (CCNY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66865-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CCNY Monoclonal antibody (66865-1-Ig)

    WB analysis of RAW 264.7 using 66865-1-Ig (same clone as 66865-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66865-1-Ig (CCNY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66865-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66865-1-PBS targets CCNY in WB, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCNY fusion protein Ag12691 相同性解析による交差性が予測される生物種
Full Name cyclin Y
Calculated molecular weight 33 kDa, 39 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC104773
Gene symbol CCNY
Gene ID (NCBI) 219771
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.