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- KD/KO Validated
CCPG1 Polyclonal antibody
CCPG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 13861-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, THP-1 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 18 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
13861-1-AP targets CCPG1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CCPG1 fusion protein Ag4841 相同性解析による交差性が予測される生物種 |
| Full Name | cell cycle progression 1 |
| Calculated molecular weight | 87 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC034914 |
| Gene symbol | CCPG1 |
| Gene ID (NCBI) | 9236 |
| RRID | AB_2074010 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cell-cycle progression gene 1 (CCPG1) is a receptor for ER stress-induced ER-phagy. CCPG1 localizes to perinuclear ER and in small foci at the ER periphery. It contains a LIR at the N-terminal cytosolic domain engaging LC3/GABARAP. (PMID: 29802216, PMID: 29290589)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCPG1 antibody 13861-1-AP | Download protocol |
| IHC protocol for CCPG1 antibody 13861-1-AP | Download protocol |
| IF protocol for CCPG1 antibody 13861-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling. | ||
Mol Cell Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.
| ||
J Pineal Res Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression | ||
Dev Cell CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.
| ||
Mol Biol Cell Lysosome Docking to WIPI1 Rings and ER-connected Phagophores Occurs During DNAJB12 and GABARAP-Dependent Selective Autophagy of Misfolded P23H-Rhodopsin. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH H (Verified Customer) (04-05-2020) | This antibody has lots of background bands. In the beginning, I was not sure which band is the right band. In the end, I performed knock-down experiment using siRNA, and validated that the band close to 98 kDa is the CCPG1 band.
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