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CCR2a-specific Polyclonal antibody

CCR2a-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16153-1-AP

Synonyms

C C chemokine receptor type 2, C C CKR 2, CC CKR 2, CCR 2, CCR2, CCR2a, CCR2a-specific, CCR2b, CD192, CKR2, CKR2A, CKR2B, CMKBR2, MCP 1 R


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Tested Applications

Positive WB detected inJurkat cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16153-1-AP targets CCR2a-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) receptor 2
Calculated molecular weight 42 kDa
Observed molecular weight35 kDa
GenBank accession numberNM_000647
Gene symbol CCR2
Gene ID (NCBI) 729230
RRIDAB_2262945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.

Protocols

Product Specific Protocols
WB protocol for CCR2a-specific antibody 16153-1-APDownload protocol
IHC protocol for CCR2a-specific antibody 16153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Thromb Res

Ferumoxytol-enhanced MRI assessment of venous Thrombus resolution and macrophage content in a murine deep vein thrombosis model

Authors - L Morrison
humanIHC

Rheumatology (Oxford)

Interleukin-6 trans-signaling regulates monocyte chemoattractant protein-1 production in immune-mediated necrotizing myopathy

Authors - Xue Ma
humanWB

Lab Invest

Matrix metalloproteinase 11 (MMP11) in macrophages promotes the migration of HER2-positive breast cancer cells and monocyte recruitment through CCL2-CCR2 signaling.

Authors - Shin Ung Kang
humanWB

Oncol Rep

CCL2/CCR2 axis induces hepatocellular carcinoma invasion and epithelial-mesenchymal transition in vitro through activation of the Hedgehog pathway.

Authors - Huijie Zhuang
ratWB

J Ethnopharmacol

Identification of potential regulating effect of baicalin on NFκB/CCL2/CCR2 signaling pathway in rats with cerebral ischemia by antibody-based array and bioinformatics analysis

Authors - Tian Xu
mouseWB,IF

Mol Immunol

QKI deficiency in macrophages protects mice against JEV infection by regulating cell migration and antiviral response.

Authors - Lele Deng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kamal Baral (Verified Customer) (01-26-2024)

Bone marrow mesenchymal stem cells from mouse stained great at the provided dilution.

  • Applications: Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse bone marrow
FH

Kamal (Verified Customer) (01-26-2024)

Bands appeared at 35 kDa. Antibody works well when diluted in 1X TBST.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse liver tissue
FH

Alexandru (Verified Customer) (11-13-2023)

Great stainig results on paraffin-embedded tissue!

  • Applications: Immunofluorescence
  • Cell Tissue Type: Mouse ventricle