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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using CCT3 Monoclonal antibody (60264-1-Ig)

    WB analysis of COLO 320 using 60264-1-Ig (same clone as 60264-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60264-1-Ig (CCT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60264-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCT3 Monoclonal antibody (60264-1-Ig)

    IHC staining of human liver cancer using 60264-1-Ig (same clone as 60264-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60264-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCT3 Monoclonal antibody (60264-1-Ig)

    IHC staining of human liver cancer using 60264-1-Ig (same clone as 60264-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60264-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CCT3 Monoclonal antibody (60264-1-Ig)

    IF Staining of HepG2 using 60264-1-Ig (same clone as 60264-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (60264-1-Ig, Clone: 5F5A6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60264-1-PBS in a different storage buffer formulation.

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CCT3 Monoclonal antibody, PBS Only

CCT3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 60264-1-PBS
Clone No.5F5A6

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CCT gamma, CCT3, CCTG, hTRiC5, PIG48, TCP 1 gamma, TRIC5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60264-1-PBS targets CCT3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CCT3 fusion protein Ag0843 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 3 (gamma)
Calculated molecular weight 60 kDa
Observed molecular weight 56-60 kDa
GenBank accession numberBC006501
Gene Symbol CCT3
Gene ID (NCBI) 7203
RRIDAB_2881385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49368
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.