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KD/KO Validated

CCT4 Polyclonal antibody

Name: CCT4 Polyclonal antibody
Brand: Proteintech
SKU: 21524-1-AP

CCT4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21524-1-AP

Synonyms

CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of HeLa using 21524-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 21524-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 21524-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 21524-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF-7 cells, mouse heart tissue, HeLa cells
Positive IHC detected in	human liver cancer tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 1 publications below

Product Information

The immunogen of 21524-1-AP is CCT4 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CCT4 fusion protein Ag16128 相同性解析による交差性が予測される生物種
Full Name	chaperonin containing TCP1, subunit 4 (delta)
Calculated molecular weight	539 aa, 58 kDa
Observed molecular weight	58-60 kDa
GenBank accession number	BC106934
Gene symbol	CCT4
Gene ID (NCBI)	10575
RRID	AB_10733520
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CCT4 antibody 21524-1-AP	Download protocol
IHC protocol for CCT4 antibody 21524-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell CCT2 is an aggrephagy receptor for clearance of solid protein aggregates. Authors - Xinyu Ma KD Validated View Article
human	WB	J Cell Biol CEP55 promotes cilia disassembly through stabilizing Aurora A kinase. Authors - Yu-Cheng Zhang View Article
human	WB	Mol Ther Nucleic Acids hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4. Authors - Qiang Luo View Article
human	WB	Pathog Dis Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT. Authors - Hirai Kimito K View Article
human	IHC	Medicine (Baltimore) Chaperonin-containing TCP-1 subunit genes are potential prognostic biomarkers and are correlated with Th2 cell infiltration in lung adenocarcinoma: An observational study Authors - Ruijuan Du View Article
human	WB	Mol Carcinog CCT4 knockdown enhances the sensitivity of cisplatin by inhibiting glycolysis in human esophageal squamous cell carcinomas Authors - Jiarui Fang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCT4 Polyclonal antibody

CCT4 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 21524-1-AP

WB analysis using 21524-1-AP

WB analysis of mouse heart using 21524-1-AP

IHC staining of human liver cancer using 21524-1-AP

IHC staining of human liver cancer using 21524-1-AP

IHC staining of human liver cancer using 21524-1-AP

IHC staining of human brain using 21524-1-AP

IHC staining of human brain using 21524-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.

CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.

hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4.

Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.

Chaperonin-containing TCP-1 subunit genes are potential prognostic biomarkers and are correlated with Th2 cell infiltration in lung adenocarcinoma: An observational study

CCT4 knockdown enhances the sensitivity of cisplatin by inhibiting glycolysis in human esophageal squamous cell carcinomas