• Featured Product
  • KD/KO Validated

CCT5 Polyclonal antibody

CCT5 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11603-1-AP

Synonyms

T-complex protein 1 subunit epsilon, KIAA0098, Chaperonin containing T-complex polypeptide 1 subunit 5, CCT-epsilon, CCTE


☆大好評トライアルサイズ ¥20,000~! >>> 一次抗体特別価格キャンペーン開催中!


Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, HEK-293 cells, A431 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11603-1-AP targets CCT5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCT5 fusion protein Ag2146 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 5 (epsilon)
Calculated molecular weight 541 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC035499
Gene symbol CCT5
Gene ID (NCBI) 22948
RRIDAB_2073774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.

Protocols

Product Specific Protocols
WB protocol for CCT5 antibody 11603-1-APDownload protocol
IHC protocol for CCT5 antibody 11603-1-APDownload protocol
IF protocol for CCT5 antibody 11603-1-APDownload protocol
IP protocol for CCT5 antibody 11603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.

Authors - Xinyu Ma
  • KD Validated
mouseWB

Nat Genet

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

Authors - Tarkar Aarti A
humanWB,IF

Proc Natl Acad Sci U S A

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Authors - Seo Seongjin S
humanWB, IF

J Cell Biol

CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.

Authors - Yu-Cheng Zhang
human,mouseWB

Front Cell Dev Biol

TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis

Authors - Wen-Shuai Tang
chickenWB,IF

Front Microbiol

Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.

Authors - Xiaohan Zhang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Andrea (Verified Customer) (01-03-2023)

Strong and clear band on the membrane.

  • Applications: Western Blot