Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, A549 cells. HeLa cells, mouse liver tissue, EC109 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
19793-1-AP targets CCT6A-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | chaperonin containing TCP1, subunit 6A (zeta 1) |
| Calculated molecular weight | 58 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | NM_001762 |
| Gene Symbol | CCT6A |
| Gene ID (NCBI) | 908 |
| RRID | AB_10638922 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40227 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCT6A, also named as CCT6, CCTZ, CCT-zeta,Tcp20 and HTR3, belongs to the TCP-1 chaperonin family. CCT6A is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. CCT6A is known to play a role, in vitro, in the folding of actin and tubulin. The antibody has no cross reaction to CCT6B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCT6A-Specific antibody 19793-1-AP | Download protocol |
| IHC protocol for CCT6A-Specific antibody 19793-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett Molecular chaperone CCT3 supports proper mitotic progression and cell proliferation in hepatocellular carcinoma cells. | ||
Mol Cell Proteomics Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities. | ||
Oncol Lett CCT6A is associated with CDC20, Enneking stage and prognosis in osteosarcoma, and its knockdown suppresses osteosarcoma cell viability and invasion | ||
Signal Transduct Target Ther Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease | ||
J Transl Med CCT6A facilitates lung adenocarcinoma progression and glycolysis via STAT1/HK2 axis |