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TCP1 eta Monoclonal antibody

TCP1 eta Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C1G1

Cat no : 67540-1-Ig

Synonyms

CCT7, CCT ETA, HIV 1 Nef interacting protein, Chaperonin containing T-complex polypeptide 1 subunit 7, CCTH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCT7 Monoclonal antibody (67540-1-Ig)

    WB analysis using 67540-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using CCT7 Monoclonal antibody (67540-1-Ig)

    WB analysis of HEK-293 using 67540-1-Ig

    WB result of CCT7 antibody (67540-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT7 transfected HEK-293 cells.

  • Western Blot (WB) analysis of NIH/3T3 cells using CCT7 Monoclonal antibody (67540-1-Ig)

    WB analysis of NIH/3T3 using 67540-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TCP1 eta Monoclonal antibody (67540-1-Ig)

    IF Staining of U2OS using 67540-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TCP1 eta Monoclonal antibody (67540-1-Ig)

    IF Staining of U2OS using 67540-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, HEK-293 cells, LNCaP cells, Hela cells, HepG cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67540-1-Ig targets TCP1 eta in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TCP1 eta fusion protein Ag9502 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 7 (eta)
Calculated molecular weight 543 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC019296
Gene symbol CCT7
Gene ID (NCBI) 10574
RRIDAB_2882759
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.

Protocols

Product Specific Protocols
WB protocol for TCP1 eta antibody 67540-1-IgDownload protocol
IF protocol for TCP1 eta antibody 67540-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Mechanism of orphan subunit recognition during assembly quality control

Authors - Yuichi Yagita
View Article