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CCT8 Polyclonal antibody
CCT8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 12263-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, mouse kidney tissue, HEK-293 cells, COLO 320 cells, HeLa cells, Jurkat cells, Raji cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | SW480 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 13 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
12263-1-AP targets CCT8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CCT8 fusion protein Ag2926 相同性解析による交差性が予測される生物種 |
| Full Name | chaperonin containing TCP1, subunit 8 (theta) |
| Calculated molecular weight | 548 aa, 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC012584 |
| Gene symbol | CCT8 |
| Gene ID (NCBI) | 10694 |
| RRID | AB_906331 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.(PMID:20080638)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCT8 antibody 12263-1-AP | Download protocol |
| IF protocol for CCT8 antibody 12263-1-AP | Download protocol |
| IP protocol for CCT8 antibody 12263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan. | ||
Proc Natl Acad Sci U S A BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Joleen (Verified Customer) (06-03-2019) | CCT8 band shows up prominently with no background.
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