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  • Featured Product
  • KD/KO Validated

CCT8 Monoclonal antibody, PBS Only

CCT8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A7C3

Cat no : 67539-1-PBS

Synonyms

C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCT8 Monoclonal antibody (67539-1-Ig)

    WB analysis using 67539-1-Ig (same clone as 67539-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67539-1-Ig (CCT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CCT8 Monoclonal antibody (67539-1-Ig)

    WB analysis of HEK-293 using 67539-1-Ig (same clone as 67539-1-PBS)

    WB result of CCT8 antibody (67539-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT8 transfected HEK-293 cells. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CCT8 Monoclonal antibody (67539-1-Ig)

    WB analysis of NIH/3T3 using 67539-1-Ig (same clone as 67539-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67539-1-Ig (CCT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCT8 Monoclonal antibody (67539-1-Ig)

    IHC staining of human liver cancer using 67539-1-Ig (same clone as 67539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67539-1-Ig (CCT8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCT8 Monoclonal antibody (67539-1-Ig)

    IHC staining of human liver cancer using 67539-1-Ig (same clone as 67539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67539-1-Ig (CCT8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCT8 Monoclonal antibody (67539-1-Ig)

    IF Staining of MCF-7 using 67539-1-Ig (same clone as 67539-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CCT8 antibody (67539-1-Ig, Clone: 1A7C3 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67539-1-PBS targets CCT8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCT8 fusion protein Ag30082 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 8 (theta)
Calculated molecular weight 548 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC012584
Gene symbol CCT8
Gene ID (NCBI) 10694
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.