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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CCZ1 Polyclonal antibody (22159-1-AP)

    WB analysis of NIH/3T3 using 22159-1-AP (same clone as 22159-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22159-1-AP (CCZ1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22159-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using CCZ1 Polyclonal antibody (22159-1-AP)

    IP experiment of NIH/3T3 using 22159-1-AP (same clone as 22159-1-PBS)

    IP result of anti-CCZ1 (IP:22159-1-AP, 4ug; Detection:22159-1-AP 1:500) with NIH/3T3 cells lysate 1500ug. This data was developed using the same antibody clone with 22159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CCZ1 Polyclonal antibody (22159-1-AP)

    IHC staining of mouse liver using 22159-1-AP (same clone as 22159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22159-1-AP (CCZ1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CCZ1 Polyclonal antibody (22159-1-AP)

    IHC staining of mouse liver using 22159-1-AP (same clone as 22159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22159-1-AP (CCZ1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22159-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22159-1-PBS targets CCZ1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCZ1 fusion protein Ag17804 相同性解析による交差性が予測される生物種
Full Name chromosome 7 open reading frame 28A
Calculated molecular weight 482 aa, 56 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC132749
Gene Symbol CCZ1
Gene ID (NCBI) 51622
RRIDAB_2879011
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP86790
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.