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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23782-1-AP

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

Validation Data Gallery

Applications:
  • IHC staining of human kidney using 23782-1-AP

    IHC staining of human kidney using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human kidney using 23782-1-AP

    IHC staining of human kidney using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human renal cell carcinoma using 23782-1-AP

    IHC staining of human renal cell carcinoma using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human renal cell carcinoma using 23782-1-AP

    IHC staining of human renal cell carcinoma using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CD10 antibody included
  • IF Staining of human kidney using 23782-1-AP

    IF Staining of human kidney using 23782-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using MME,CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human kidney using 23782-1-AP

    IF Staining of human kidney using 23782-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using MME,CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Jurkat using 23782-1-AP

    FC experiment of Jurkat using 23782-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MME,CD10 (23782-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

23782-1-AP targets MME,CD10 in IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MME,CD10 fusion protein Ag20634 相同性解析による交差性が予測される生物種
Full Name membrane metallo-endopeptidase
Calculated molecular weight 750 aa, 86 kDa
GenBank accession numberBC101632
Gene symbol MME
Gene ID (NCBI) 4311
RRIDAB_2879323
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Protocols

Product Specific Protocols
IHC protocol for MME,CD10 antibody 23782-1-APDownload protocol
IF protocol for MME,CD10 antibody 23782-1-APDownload protocol
FC protocol for MME,CD10 antibody 23782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols