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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Neprilysin/CD10 Polyclonal antibody

Neprilysin/CD10 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 23782-1-AP

Synonyms

MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (23782-1-AP)

    IHC staining of human kidney using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (Neprilysin/CD10 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (23782-1-AP)

    IHC staining of human kidney using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (Neprilysin/CD10 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using MME,CD10 Polyclonal antibody (23782-1-AP)

    IHC staining of human renal cell carcinoma using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using MME,CD10 Polyclonal antibody (23782-1-AP)

    IHC staining of human renal cell carcinoma using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (23782-1-AP)

    IF Staining of human kidney using 23782-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Neprilysin/CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (23782-1-AP)

    IF Staining of human kidney using 23782-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Neprilysin/CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

23782-1-AP targets Neprilysin/CD10 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neprilysin/CD10 fusion protein Ag20634 相同性解析による交差性が予測される生物種
Full Name membrane metallo-endopeptidase
Calculated molecular weight 750 aa, 86 kDa
GenBank accession numberBC101632
Gene symbol CD10
Gene ID (NCBI) 4311
RRIDAB_2879323
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

Protocols

Product Specific Protocols
IHC protocol for Neprilysin/CD10 antibody 23782-1-APDownload protocol
IF protocol for Neprilysin/CD10 antibody 23782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Clin Breast Cancer

Inhibitory Impact Of Cinobufagin In Triple-Negative Breast Cancer Metastasis: Involvements Of Macrophage Reprogramming Through Upregulated MME and Inactivated FAK/STAT3 Signaling

Authors - Zhaohui Zhu
View Article