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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody, PBS Only

Neprilysin/CD10 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241322E1

Cat no : 98116-1-PBS

Synonyms

MME, Neprilysin, Enkephalinase, EC:3.4.24.11, CD10

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human Neprilysin/CD10 Rabbit Recombinant Anti (98116-1-RR)

    FC experiment of human peripheral blood leukocytes using 98116-1-RR (same clone as 98116-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone: 241322E1) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98116-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Reh cells using Anti-Human Neprilysin/CD10 Rabbit Recombinant Anti (98116-1-RR)

    FC experiment of Reh using 98116-1-RR (same clone as 98116-1-PBS)

    1x10^6 Reh cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone: 241322E1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98116-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human Neprilysin/CD10 Rabbit Recombinant Anti (98116-1-RR)

    FC experiment of human peripheral blood leukocytes using 98116-1-RR (same clone as 98116-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone:241322E1)(red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98116-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98116-1-RR

    Affinity and Kinetic Characterization of 98116-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98116-1-RR against Human CD10 were performed. The affinity constant is 9.9 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98116-1-PBS targets Neprilysin/CD10 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name membrane metallo-endopeptidase
Calculated molecular weight 750 aa, 86 kDa
GenBank accession numberBC101658
Gene symbol CD10
Gene ID (NCBI) 4311
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).