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  • Featured Product
  • KD/KO Validated

CD100 Monoclonal antibody, PBS Only

CD100 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C1A6

Cat no : 66582-1-PBS

Synonyms

A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD100 Monoclonal antibody (66582-1-Ig)

    WB analysis using 66582-1-Ig (same clone as 66582-1-PBS)

    various cells were subjected to SDS PAGE followed by western blot with 66582-1-Ig (CD100 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CD100 Monoclonal antibody (66582-1-Ig)

    WB analysis of HeLa using 66582-1-Ig (same clone as 66582-1-PBS)

    WB result of CD100 antibody (66582-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD100 transfected HeLa cells. This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD100 Monoclonal antibody (66582-1-Ig)

    IHC staining of human tonsillitis using 66582-1-Ig (same clone as 66582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD100 Monoclonal antibody (66582-1-Ig)

    IHC staining of human tonsillitis using 66582-1-Ig (same clone as 66582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD100 Monoclonal antibody (66582-1-Ig)

    IHC staining of human lymphoma using 66582-1-Ig (same clone as 66582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD100 Monoclonal antibody (66582-1-Ig)

    IHC staining of human lymphoma using 66582-1-Ig (same clone as 66582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CD100 Monoclonal antibody (66582-1-Ig)

    IHC staining of human kidney using 66582-1-Ig (same clone as 66582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CD100 Monoclonal antibody (66582-1-Ig)

    IHC staining of human kidney using 66582-1-Ig (same clone as 66582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD100 Monoclonal antibody (66582-1-Ig)

    IHC staining of human appendicitis using 66582-1-Ig (same clone as 66582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD100 Monoclonal antibody (66582-1-Ig)

    IHC staining of human appendicitis using 66582-1-Ig (same clone as 66582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66582-1-PBS targets CD100 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD100 fusion protein Ag26461 相同性解析による交差性が予測される生物種
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Calculated molecular weight 96 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC054500
Gene symbol SEMA4D
Gene ID (NCBI) 10507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.